The University of Queensland

Cell-intrinsic C5a synergizes with Dectin-1 in macrophages to mediate fungal killing

Li, Xaria X., Fung, Jenny N., Clark, Richard J., Lee, John D. and Woodruff, Trent M. (2024). Cell-intrinsic C5a synergizes with Dectin-1 in macrophages to mediate fungal killing. Proceedings of the National Academy of Sciences, 121 (5), e2314627121. doi: 10.1073/pnas.2314627121

Protocol for cell-based screening assay to measure ERK1/2 phosphorylation as a readout for complement receptor activation

Li, Xaria X. and Woodruff, Trent M. (2023). Protocol for cell-based screening assay to measure ERK1/2 phosphorylation as a readout for complement receptor activation. STAR Protocols, 4 (4) 102758, 102758. doi: 10.1016/j.xpro.2023.102758

Molecular basis of anaphylatoxin binding, activation, and signaling bias at complement receptors

Yadav, Manish K., Maharana, Jagannath, Yadav, Ravi, Saha, Shirsha, Sarma, Parishmita, Soni, Chahat, Singh, Vinay, Saha, Sayantan, Ganguly, Manisankar, Li, Xaria X., Mohapatra, Samanwita, Mishra, Sudha, Khant, Htet A., Chami, Mohamed, Woodruff, Trent M., Banerjee, Ramanuj, Shukla, Arun K. and Gati, Cornelius (2023). Molecular basis of anaphylatoxin binding, activation, and signaling bias at complement receptors. Cell, 186 (22), 4956-4973.e21. doi: 10.1016/j.cell.2023.09.020

The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors

Alexander, Stephen P. H., Christopoulos, Arthur, Davenport, Anthony P., Kelly, Eamonn, Mathie, Alistair A., Peters, John A., Veale, Emma L., Armstrong, Jane F., Faccenda, Elena, Harding, Simon D., Davies, Jamie A., Abbracchio, Maria Pia, Abraham, George, Agoulnik, Alexander, Alexander, Wayne, Al‐hosaini, Khaled, Bäck, Magnus, Baker, Jillian G., Barnes, Nicholas M., Bathgate, Ross, Beaulieu, Jean‐Martin, Beck‐Sickinger, Annette G., Behrens, Maik, Bernstein, Kenneth E., Bettler, Bernhard, Birdsall, Nigel J. M., Blaho, Victoria, Boulay, Francois, Bousquet, Corinne ... Ye, Richard D. (2023). The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors. British Journal of Pharmacology, 180 (S2), S23-S144. doi: 10.1111/bph.16177

Complement peptide receptors in GtoPdb v.2023.1

Cianciulli, Antonia, Coulthard, Liam, Hawksworth, Owen, Lee, John D., Li, Xaria X., Mitolo, Vincenzo, Monk, Peter, Panaro, Maria A. and Woodruff, Trent M. (2023). Complement peptide receptors in GtoPdb v.2023.1. IUPHAR/BPS Guide to Pharmacology CITE, 2023 (1). doi: 10.2218/gtopdb/f5/2023.1

TLQP-21 is a low potency partial C3aR activator on human primary macrophages

Li, Xaria X., Lee, John D., Lee, Han S., Clark, Richard J. and Woodruff, Trent M. (2023). TLQP-21 is a low potency partial C3aR activator on human primary macrophages. Frontiers in Immunology, 14 1086673, 1-10. doi: 10.3389/fimmu.2023.1086673

SARS-CoV-2 drives NLRP3 inflammasome activation in human microglia through spike protein

Albornoz, Eduardo A., Amarilla, Alberto A., Modhiran, Naphak, Parker, Sandra, Li, Xaria X., Wijesundara, Danushka K., Aguado, Julio, Zamora, Adriana Pliego, McMillan, Christopher L. D., Liang, Benjamin, Peng, Nias Y. G., Sng, Julian D. J., Saima, Fatema Tuj, Fung, Jenny N., Lee, John D., Paramitha, Devina, Parry, Rhys, Avumegah, Michael S., Isaacs, Ariel, Lo, Martin W., Miranda-Chacon, Zaray, Bradshaw, Daniella, Salinas-Rebolledo, Constanza, Rajapakse, Niwanthi W., Wolvetang, Ernst J., Munro, Trent P., Rojas-Fernandez, Alejandro, Young, Paul R., Stacey, Katryn J. ... Woodruff, Trent M. (2023). SARS-CoV-2 drives NLRP3 inflammasome activation in human microglia through spike protein. Molecular Psychiatry, 28 (7), 2878-2893. doi: 10.1038/s41380-022-01831-0

Success in navigating hurdles to oral delivery of a bioactive peptide complement antagonist through use of nanoparticles to increase bioavailability and in vivo efficacy

Xu, Weizhi, Kumar, Vinod, Cui, Cedric S., Li, Xaria X., Whittaker, Andrew K., Xu, Zhi Ping, Smith, Maree T., Woodruff, Trent M. and Han, Felicity Y (2022). Success in navigating hurdles to oral delivery of a bioactive peptide complement antagonist through use of nanoparticles to increase bioavailability and in vivo efficacy. Advanced Therapeutics, 5 (12) 2200109, 2200109. doi: 10.1002/adtp.202200109

Unexpected off-target activities for recombinant C5a in human macrophages

Li, Xaria X., Gorman, Declan M., Lee, John D., Clark, Richard J. and Woodruff, Trent M. (2021). Unexpected off-target activities for recombinant C5a in human macrophages. Journal of Immunology, 208 (1), 133-142. doi: 10.4049/jimmunol.2100444

Development of synthetic human and mouse C5a: application to binding and functional assays in vitro and in vivo

Gorman, Declan M., Li, Xaria X., Payne, Colton D., Cui, Cedric S., Lee, John D., Rosengren, K. Johan, Woodruff, Trent M. and Clark, Richard J. (2021). Development of synthetic human and mouse C5a: application to binding and functional assays in vitro and in vivo. ACS Pharmacology and Translational Science, 4 (6) acsptsci.1c00199, 1808-1817. doi: 10.1021/acsptsci.1c00199

Development of Potent and Selective Agonists for Complement C5a Receptor 1 with In Vivo Activity

Gorman, Declan M., Li, Xaria X., Lee, John D., Fung, Jenny N., Cui, Cedric S., Lee, Han Siean, Rolfe, Barbara E., Woodruff, Trent M. and Clark, Richard J. (2021). Development of Potent and Selective Agonists for Complement C5a Receptor 1 with In Vivo Activity. Journal of Medicinal Chemistry, 64 (22) acs.jmedchem.1c01174, 16598-16608. doi: 10.1021/acs.jmedchem.1c01174

Anaphylatoxin receptor promiscuity for commonly used complement C5a peptide agonists

Li, Xaria X., Clark, Richard J. and Woodruff, Trent M. (2021). Anaphylatoxin receptor promiscuity for commonly used complement C5a peptide agonists. International Immunopharmacology, 100 108074, 108074. doi: 10.1016/j.intimp.2021.108074

Intrinsic bias at non-canonical, β-arrestin-coupled seven transmembrane receptors

Pandey, Shubhi, Kumari, Punita, Baidya, Mithu, Kise, Ryoji, Cao, Yubo, Dwivedi-Agnihotri, Hemlata, Banerjee, Ramanuj, Li, Xaria X., Cui, Cedric S., Lee, John D., Kawakami, Kouki, Maharana, Jagannath, Ranjan, Ashutosh, Chaturvedi, Madhu, Jhingan, Gagan Deep, Laporte, Stéphane A., Woodruff, Trent M., Inoue, Asuka and Shukla, Arun K. (2021). Intrinsic bias at non-canonical, β-arrestin-coupled seven transmembrane receptors. Molecular Cell, 81 (22), 4605-4621.e11. doi: 10.1016/j.molcel.2021.09.007

Optimisation of a microfluidic method for the delivery of a small peptide

Han, Felicity Y., Xu, Weizhi, Kumar, Vinod, Cui, Cedric S., Li, Xaria, Jiang, Xingyu, Woodruff, Trent M., Whittaker, Andrew K. and Smith, Maree T. (2021). Optimisation of a microfluidic method for the delivery of a small peptide. Pharmaceutics, 13 (9) 1505, 1505. doi: 10.3390/pharmaceutics13091505

The concise guide to pharmacology 2021/22: G protein‐coupled receptors

Alexander, Stephen PH, Christopoulos, Arthur, Davenport, Anthony P, Kelly, Eamonn, Mathie, Alistair, Peters, John A, Veale, Emma L, Armstrong, Jane F, Faccenda, Elena, Harding, Simon D, Pawson, Adam J, Southan, Christopher, Davies, Jamie A, Abbracchio, Maria Pia, Alexander, Wayne, Al‐hosaini, Khaled, Bäck, Magnus, Barnes, Nicholas M., Bathgate, Ross, Beaulieu, Jean‐Martin, Bernstein, Kenneth E., Bettler, Bernhard, Birdsall, Nigel J.M., Blaho, Victoria, Boulay, Francois, Bousquet, Corinne, Bräuner‐Osborne, Hans, Burnstock, Geoffrey, Caló, Girolamo ... Ye, Richard D. (2021). The concise guide to pharmacology 2021/22: G protein‐coupled receptors. British Journal of Pharmacology, 178 (S1), S27-S156. doi: 10.1111/bph.15538

Complement peptide receptors in GtoPdb v.2021.3

Cianciulli, Antonia, Coulthard, Liam, Hawksworth, Owen, Lee, John D., Li, Xaria X., Mitolo, Vincenzo, Monk, Peter, Panaro, Maria A. and Woodruff, Trent M. (2021). Complement peptide receptors in GtoPdb v.2021.3. IUPHAR/BPS Guide to Pharmacology CITE, 2021 (3). doi: 10.2218/gtopdb/f5/2021.3

The “C3aR Antagonist” SB290157 is a partial C5aR2 agonist

Li, Xaria X., Kumar, Vinod, Clark, Richard J., Lee, John D. and Woodruff, Trent M. (2021). The “C3aR Antagonist” SB290157 is a partial C5aR2 agonist. Frontiers in Pharmacology, 11 591398, 591398. doi: 10.3389/fphar.2020.591398

Pharmacological characterisation of small molecule C5aR1 inhibitors in human cells reveals biased activities for signalling and function

Li, Xaria X., Lee, John D., Massey, Nicholas L., Guan, Carolyn, Robertson, Avril A. B., Clark, Richard J. and Woodruff, Trent M. (2020). Pharmacological characterisation of small molecule C5aR1 inhibitors in human cells reveals biased activities for signalling and function. Biochemical Pharmacology, 180 114156, 114156. doi: 10.1016/j.bcp.2020.114156

C5aR2 activation broadly modulates the signaling and function of primary human macrophages

Li, Xaria X., Clark, Richard J. and Woodruff, Trent M. (2020). C5aR2 activation broadly modulates the signaling and function of primary human macrophages. Journal of Immunology, 205 (4), 1-11. doi: 10.4049/jimmunol.2000407

Emerging insights into the structure and function of complement C5a receptors

Pandey, Shubhi, Maharana, Jagannath, Li, Xaria X., Woodruff, Trent M. and Shukla, Arun K. (2020). Emerging insights into the structure and function of complement C5a receptors. Trends in Biochemical Sciences, 45 (8), 693-705. doi: 10.1016/j.tibs.2020.04.004

C5a receptors C5aR1 and C5aR2 mediate opposing pathologies in a mouse model of melanoma

Nabizadeh, Jamileh A., Manthey, Helga D., Panagides, Nadya, Steyn, Frederik J., Lee, John D., Li, Xaria X., Akhir, Fazrena N. M., Chen, Weiyu, Boyle, Glen M., Taylor, Stephen M., Woodruff, Trent M. and Rolfe, Barbara E. (2019). C5a receptors C5aR1 and C5aR2 mediate opposing pathologies in a mouse model of melanoma. FASEB Journal, 33 (10), fj201800980RR-11071. doi: 10.1096/fj.201800980rr

The complement receptor C5aR2: a powerful modulator of innate and adaptive immunity

Li, Xaria X., Lee, John D., Kemper, Claudia and Woodruff, Trent M. (2019). The complement receptor C5aR2: a powerful modulator of innate and adaptive immunity. Journal of Immunology, 202 (12), 3339-3348. doi: 10.4049/jimmunol.1900371

Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2

Brennan, Faith H., Jogia, Trisha, Gillespie, Ellen R., Blomster, Linda V., Li, Xaria X., Nowlan, Bianca, Williams, Gail M., Jacobson, Esther, Osborne, Geoff W., Meunier, Frederic A., Taylor, Stephen M., Campbell, Kate E., MacDonald, Kelli P.A., Levesque, Jean-Pierre, Woodruff, Trent M. and Ruitenberg, Marc J. (2019). Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2. JCI Insight, 4 (9) e98254. doi: 10.1172/jci.insight.98254

Partial ligand-receptor engagement yields functional bias at the human complement receptor, C5aR1

Pandey, Shubhi, Li, Xaria X., Srivastava, Ashish, Baidya, Mithu, Kumari, Punita, Dwivedi, Hemlata, Chaturvedi, Madhu, Ghosh, Eshan, Woodruff, Trent M. and Shukla, Arun K. (2019). Partial ligand-receptor engagement yields functional bias at the human complement receptor, C5aR1. Journal of Biological Chemistry, 294 (24), jbc.RA119.007485-9429. doi: 10.1074/jbc.ra119.007485

New concepts on the therapeutic control of complement anaphylatoxin receptors

Hawksworth, Owen A., Li, Xaria X., Coulthard, Liam G., Wolvetang, Ernst J. and Woodruff, Trent M. (2017). New concepts on the therapeutic control of complement anaphylatoxin receptors. Molecular Immunology, 89, 36-43. doi: 10.1016/j.molimm.2017.05.015

Complement C5a receptors, C5aR1 and C5aR2, mediate opposing pathologies in a mouse model of melanoma

Nabizadeh, J., Manthey, H., Panagides, N., Steyn, F., Lee, J., Li, X., Woodruff, T. and Rolfe, B. (2019). Complement C5a receptors, C5aR1 and C5aR2, mediate opposing pathologies in a mouse model of melanoma. 17th International Congress of Immunology (IUIS), Beijing, China, 19-23 October 2019. Weinheim, Germany: Wiley.

Investigating the immunopharmacology of the complement C5a receptors, C5aR1 and C5aR2

Li, Xiang (2021). Investigating the immunopharmacology of the complement C5a receptors, C5aR1 and C5aR2. PhD Thesis, Faculty of Medicine, The University of Queensland. doi: 10.14264/339adea