The University of Queensland

A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments

Li, Junrong, Howard, Christopher B., Dey, Shuvashis, Lowry, Kym, Whiley, David M., Puttick, Simon, Rose, Stephen, Lobb, Richard J., Wuethrich, Alain, Edwardraja, Selvakumar and Trau, Matt (2023). A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments. Nature Nanotechnology, 18 (10), 1222-1229. doi: 10.1038/s41565-023-01415-1

Exploring performance parameters of artificial allosteric protein switches

Ergun Ayva, Cagla, Fiorito, Maria M., Guo, Zhong, Edwardraja, Selvakumar, Kaczmarski, Joe A., Gagoski, Dejan, Walden, Patricia, Johnston, Wayne A., Jackson, Colin J., Nebl, Tom and Alexandrov, Kirill (2022). Exploring performance parameters of artificial allosteric protein switches. Journal of Molecular Biology, 434 (17) 167678, 1-14. doi: 10.1016/j.jmb.2022.167678

Engineering and exploiting synthetic allostery of NanoLuc luciferase

Guo, Zhong, Parakra, Rinky D., Xiong, Ying, Johnston, Wayne A., Walden, Patricia, Edwardraja, Selvakumar, Moradi, Shayli Varasteh, Ungerer, Jacobus P. J., Ai, Hui-wang, Phillips, Jonathan J. and Alexandrov, Kirill (2022). Engineering and exploiting synthetic allostery of NanoLuc luciferase. Nature Communications, 13 (1) 789, 789. doi: 10.1038/s41467-022-28425-2

Generation of nanoyeast single-chain variable fragments as high-avidity biomaterials for dengue virus detection

Farokhinejad, Fahimeh, Lane, Rebecca E., Lobb, Richard J., Edwardraja, Selvakumar, Wuethrich, Alain, Howard, Christopher B. and Trau, Matt (2021). Generation of nanoyeast single-chain variable fragments as high-avidity biomaterials for dengue virus detection. ACS Biomaterials Science and Engineering, 7 (12) acsbiomaterials.1c01001, 5850-5860. doi: 10.1021/acsbiomaterials.1c01001

Connecting artificial proteolytic and electrochemical signaling systems with caged messenger peptides

Bollella, Paolo, Edwardraja, Selvakumar, Guo, Zhong, Vickers, Claudia E., Whitfield, Jason, Walden, Patricia, Melman, Artem, Alexandrov, Kirill and Katz, Evgeny (2021). Connecting artificial proteolytic and electrochemical signaling systems with caged messenger peptides. ACS Sensors, 6 (10), 3596-3603. doi: 10.1021/acssensors.1c00845

Amplification-free SARS-CoV-2 detection using nanoyeast-scFv and ultrasensitive plasmonic nanobox-integrated nanomixing microassay

Li, Junrong, Wuethrich, Alain, Edwardraja, Selvakumar, Lobb, Richard J., Puttick, Simon, Rose, Stephen, Howard, Christopher B. and Trau, Matt (2021). Amplification-free SARS-CoV-2 detection using nanoyeast-scFv and ultrasensitive plasmonic nanobox-integrated nanomixing microassay. Analytical Chemistry, 93 (29) acs.analchem.1c01657, 10251-10260. doi: 10.1021/acs.analchem.1c01657

Self-powered molecule release systems activated with chemical signals processed through reconfigurable Implication or Inhibition Boolean logic gates

Bollella, Paolo, Guo, Zhong, Edwardraja, Selvakumar, Krishna Kadambar, Vasantha, Alexandrov, Kirill, Melman, Artem and Katz, Evgeny (2021). Self-powered molecule release systems activated with chemical signals processed through reconfigurable Implication or Inhibition Boolean logic gates. Bioelectrochemistry, 138 107735, 107735. doi: 10.1016/j.bioelechem.2020.107735

Control of allosteric electrochemical protein switch using magnetic signals

Bollella, Paolo, Edwardraja, Selvakumar, Guo, Zhong, Alexandrov, Kirill and Katz, Evgeny (2020). Control of allosteric electrochemical protein switch using magnetic signals. Chemical Communications, 56 (64), 9206-9209. doi: 10.1039/d0cc04284f

Control of Allosteric Protein Electrochemical Switches with Biomolecular and Electronic Signals

Bollella, Paolo, Edwardraja, Selvakumar, Guo, Zhong, Alexandrov, Kirill and Katz, Evgeny (2020). Control of Allosteric Protein Electrochemical Switches with Biomolecular and Electronic Signals. The journal of physical chemistry letters, 11 (14), 5549-5554. doi: 10.1021/acs.jpclett.0c01223

Caged activators of artificial allosteric protein biosensors

Edwardraja, Selvakumar, Guo, Zhong, Whitfield, Jason, Lantadilla, Ignacio Retamal, Johnston, Wayne A., Walden, Patricia, Vickers, Claudia E. and Alexandrov, Kirill (2020). Caged activators of artificial allosteric protein biosensors. ACS Synthetic Biology, 9 (6) acssynbio.9b00500, 1306-1314. doi: 10.1021/acssynbio.9b00500

Generalizable protein biosensors based on synthetic switch modules

Guo, Zhong, Johnston, Wayne A., Whitfield, Jason, Walden, Patricia, Cui, Zhenling, Wijker, Elvira, Edwardraja, Selvakumar, Retamal Lantadilla, Ignacio, Ely, Fernanda, Vickers, Claudia, Ungerer, Jacobus P. J. and Alexandrov, Kirill (2019). Generalizable protein biosensors based on synthetic switch modules. Journal of the American Chemical Society, 141 (20) jacs.8b12298, 8128-8135. doi: 10.1021/jacs.8b12298

Computational screening of potential non-immunoglobulin scaffolds using overlapped conserved residues (OCR)-based fingerprints

Munussami, Ganapathiraman, Sokalingam, Sriram, Edwardraja, Selvakumar, Kim, Jung Rae, Chung, Sungwook and Lee, Sun-Gu (2018). Computational screening of potential non-immunoglobulin scaffolds using overlapped conserved residues (OCR)-based fingerprints. Korean Journal of Chemical Engineering, 35 (3), 717-724. doi: 10.1007/s11814-017-0350-4

Biochemical and spectroscopic characterization of dinuclear Mn-sites in artificial four-helix bundle proteins

Olson, Tien L., Espiritu, Eduardo, Edwardraja, Selvakumar, Canarie, Elizabeth, Flores, Marco, Williams, JoAnn C., Ghirlanda, Giovanna and Allen, James P. (2017). Biochemical and spectroscopic characterization of dinuclear Mn-sites in artificial four-helix bundle proteins. Biochimica et Biophysica Acta - Bioenergetics, 1858 (12), 945-954. doi: 10.1016/j.bbabio.2017.08.013

Rational design of an anticalin-type sugar-binding protein using a genetically encoded boronate side chain

Edwardraja, Selvakumar, Eichinger, Andreas, Theobald, Ina, Sommer, Carina Andrea, Reichert, Andreas J and Skerra, Arne (2017). Rational design of an anticalin-type sugar-binding protein using a genetically encoded boronate side chain. ACS Synthetic Biology, 6 (12), 2241-2247. doi: 10.1021/acssynbio.7b00199

Generation of efficient fingerprint for GFP-like fold and computational identification of potential GFP-like homologs

Edwardraja, Selvakumar, Munussami, Ganapathiraman, Goyal, Amit and Lee, Sun-Gu (2016). Generation of efficient fingerprint for GFP-like fold and computational identification of potential GFP-like homologs. Biotechnology and Bioprocess Engineering, 21 (6), 712-719. doi: 10.1007/s12257-016-0362-7

Design of dinuclear manganese cofactors for bacterial reaction centers

Olson, Tien L., Espiritu, Eduardo, Edwardraja, Selvakumar, Simmons, Chad R., Williams, JoAnn C., Ghirlanda, Giovanna and Allen, James P. (2016). Design of dinuclear manganese cofactors for bacterial reaction centers. Biochimica et Biophysica Acta - Bioenergetics, 1857 (5), 539-547. doi: 10.1016/j.bbabio.2015.09.003

Copper environment in artificial metalloproteins probed by electron paramagnetic resonance spectroscopy

Flores, Marco, Olson, Tien L., Wang, Dong, Edwardraja, Selvakumar, Shinde, Sandip, Williams, JoAnn C., Ghirlanda, Giovanna and Allen, James P. (2015). Copper environment in artificial metalloproteins probed by electron paramagnetic resonance spectroscopy. Journal of Physical Chemistry B, 119 (43), 13825-13833. doi: 10.1021/acs.jpcb.5b04172

Targeted delivery of ubiquitin-conjugated BH3 peptide-based Mcl-1 inhibitors into cancer cells

Muppidi, Avinash, Doi, Kenichiro, Edwardraja, Selvakumar, Pulayarti, Surya V. S. R. K., Szyperski, Thomas, Wang, Hong-Gang and Lin, Qing (2014). Targeted delivery of ubiquitin-conjugated BH3 peptide-based Mcl-1 inhibitors into cancer cells. Bioconjugate Chemistry, 25 (2), 424-432. doi: 10.1021/bc4005574

3-Hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid

Lee, Philah, Raj, Subramanian Mohan, Zhou, Shengfang, Ashok, Somasundar, Edwardraja, Selvakumar and Park, Sunghoon (2014). 3-Hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid. Biotechnology and Bioprocess Engineering, 19 (1), 1-7. doi: 10.1007/s12257-013-0487-x

Cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from Pseudomonas denitrificans ATCC 13867

Zhou, Shengfang, Raj, Subramanian Mohan, Ashok, Somasundar, Edwardraja, Selvakumar, Lee, Sun-gu and Park, Sunghoon (2013). Cloning, expression and characterization of 3-hydroxyisobutyrate dehydrogenase from Pseudomonas denitrificans ATCC 13867. PLoS ONE, 8 (5) e62666, e62666. doi: 10.1371/journal.pone.0062666

Generation of anti-c-Met single domain antibody fragment based on human stable frameworks

Edwardraja, Selvakumar, Sokalingam, Sriram, Raghunathan, Govindan, Hwang, Bum-Yeol and Lee, Sun-Gu (2012). Generation of anti-c-Met single domain antibody fragment based on human stable frameworks. Biotechnology and Bioprocess Engineering, 17 (6), 1120-1127. doi: 10.1007/s12257-012-0378-6

Rational design of proteolytically stable, cell-permeable peptide-based selective Mcl-1 inhibitors

Muppidi, Avinash, Doi, Kenichiro, Edwardraja, Selvakumar, Drake, Eric J., Gulick, Andrew M., Wang, Hong-Gang and Lin, Qing (2012). Rational design of proteolytically stable, cell-permeable peptide-based selective Mcl-1 inhibitors. Journal of the American Chemical Society, 134 (36), 14734-14737. doi: 10.1021/ja306864v

Enhancing the productivity of soluble green fluorescent protein through methionine-residue specific consensus approach

Soundrarajan, Nagasundarapandian, Edwardraja, Selvakumar, Lee, Sun-Gu, Yun, Hyungdon and Ayyadurai, Niraikulam (2012). Enhancing the productivity of soluble green fluorescent protein through methionine-residue specific consensus approach. African Journal of Biotechnology, 11 (5), 1059-1064. doi: 10.5897/AJB11.3816

Copper-free sonogashira cross-coupling for functionalization of alkyne-encoded proteins in aqueous medium and in bacterial cells

Li, Nan, Lim, Reyna K. V., Edwardraja, Selvakumar and Lin, Qing (2011). Copper-free sonogashira cross-coupling for functionalization of alkyne-encoded proteins in aqueous medium and in bacterial cells. Journal of the American Chemical Society, 133 (39), 15316-15319. doi: 10.1021/ja2066913

Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues

Edwardraja, Selvakumar, Sriram, Sokalingam, Govindan, Raghunathan, Budisa, Nediljko and Lee, Sun-Gu (2011). Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues. Molecular BioSystems, 7 (1), 258-265. doi: 10.1039/c0mb00154f

Redesigning of anti-c-Met single chain Fv antibody for the cytoplasmic folding and its structural analysis

Edwardraja, Selvakumar, Neelamegam, Rameshkumar, Ramadoss, Vijayaraj, Venkatesan, Subramanian and Lee, Sun-Gu (2010). Redesigning of anti-c-Met single chain Fv antibody for the cytoplasmic folding and its structural analysis. Biotechnology and Bioengineering, 106 (3), 367-375. doi: 10.1002/bit.22702

In vivo production of functional single-chain Fv fragment with an N-terminal-specific bio-orthogonal reactive group

Selvakumar, Edwardraja, Rameshkumar, Neelamegam, Lee, Sun-Gu, Lee, Soo-Jae and Park, Hyung-Soon (2010). In vivo production of functional single-chain Fv fragment with an N-terminal-specific bio-orthogonal reactive group. ChemBioChem, 11 (4), 498-501. doi: 10.1002/cbic.200900685

A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate

Raj, Subramanian Mohan, Rathnasingh, Chelladurai, Jung, Woo-Chel, Selvakumar, Edwardraja and Park, Sunghoon (2010). A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate. Biotechnology and Bioprocess Engineering, 15 (1), 131-138. doi: 10.1007/s12257-010-0030-2

Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli

Ayyadurai, Niraikulam, Neelamegam, Rameshkumar, Nagasundarapandian, Soundrarajan, Edwardraja, Selvakumar, Park, Hyung Soon, Lee, Soo Jae, Yoo, Tae Hyeon, Yoon, Hyungdon and Lee, Sun-Gu (2009). Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli. Biotechnology and Bioprocess Engineering, 14 (3), 257-265. doi: 10.1007/s12257-009-0009-z

Cloning, expression, and characterization of an aldehyde dehydrogenase from Escherichia coli K-12 that utilizes 3-Hydroxypropionaldehyde as a substrate

Jo, Ji-Eun, Raj, Subramanian Mohan, Rathnasingh, Chelladurai, Selvakumar, Edwardraja, Jung, Woo-Chel and Park, Sunghoon (2008). Cloning, expression, and characterization of an aldehyde dehydrogenase from Escherichia coli K-12 that utilizes 3-Hydroxypropionaldehyde as a substrate. Applied Microbiology and Biotechnology, 81 (1), 51-60. doi: 10.1007/s00253-008-1608-x

Improving the productivity of single-chain Fv antibody against c-Met by rearranging the order of its variable domains

Kim, Yu-Jin, Neelamegam, Rameshkumar, Heo, Mi-Ae, Edwardraja, Selvakumar, Paik, Hyun-Jong and Lee, Sun-Gu (2008). Improving the productivity of single-chain Fv antibody against c-Met by rearranging the order of its variable domains. Journal of Microbiology and Biotechnology, 18 (6), 1186-1190.

Connecting biochemistry and elelctronics with artifical allosteric protein biosensors

Guo, Zhong, Whitfield, Jason, Edwardraja, Selvakumar and Alexandrov, Kirill (2018). Connecting biochemistry and elelctronics with artifical allosteric protein biosensors. 2018 International Conference Laser Optics, ICLO 2018, St. Petersburg, Russia, 4-8 June 2018. Piscataway, NJ, United States: Institute of Electrical and Electronics Engineers. doi: 10.1109/LO.2018.8435862