Journal Article: Kinetics of severe dengue virus infection and development of gut pathology in mice
Pliego Zamora, Adriana, Kim, Jaehyeon, Vajjhala, Parimala R., Thygesen, Sara J., Watterson, Daniel, Modhiran, Naphak, Bielefeldt-Ohmann, Helle and Stacey, Katryn J. (2023). Kinetics of severe dengue virus infection and development of gut pathology in mice. Journal of Virology, 97 (11) ARTN e0125123, e0125123. doi: 10.1128/jvi.01251-23
Journal Article: TLR4 phosphorylation at tyrosine 672 activates the ERK/c‐FOS signaling module for LPS‐induced cytokine responses in macrophages
Curson, James E. B., Liu, Liping, Luo, Lin, Muusse, Timothy W., Lucas, Richard M., Gunther, Kimberley S., Vajjhala, Parimala R., Abrol, Rishika, Jones, Alun, Kapetanovic, Ronan, Stacey, Katryn J., Stow, Jennifer L. and Sweet, Matthew J. (2023). TLR4 phosphorylation at tyrosine 672 activates the ERK/c‐FOS signaling module for LPS‐induced cytokine responses in macrophages. European Journal of Immunology, 53 (7) e2250056, e2250056. doi: 10.1002/eji.202250056
Journal Article: Flow cytometric reporter assays provide robust functional analysis of signaling complexes
Muusse, Timothy W., Lee, Morris Y.L., Kim, Hyoyoung, Parat, Marie-Odile, Nanson, Jeffrey D., Kobe, Bostjan, Vajjhala, Parimala R. and Stacey, Katryn J. (2022). Flow cytometric reporter assays provide robust functional analysis of signaling complexes. Journal of Biological Chemistry, 298 (12) 102666, 102666. doi: 10.1016/j.jbc.2022.102666
Mammalian endotoxin: Characterisation of highly inflammatory endogenous material
(2021–2024) NHMRC IDEAS Grants
Molecular analysis of TLR4 signalling
(2023) Doctor Philosophy
Toll-like receptor signalling mechanisms
Doctor Philosophy
Structural and functional analysis of TIR domain enzymatic activity
Doctor Philosophy
Assessment of inflammasome formation by flow cytometry
Sester, David P., Zamoshnikova, Alina, Thygesen, Sara J., Vajjhala, Parimala R., Cridland, Simon O., Schroder, Kate and Stacey, Katryn J. (2016). Assessment of inflammasome formation by flow cytometry. Current protocols in immunology. (pp. 14.40.1-14.40.29) edited by Coligan, John E., Bierer, Barbara, Margulies, David H., Shevach, Ethan M. and Strober, Warren. Hoboken, NJ United States: John Wiley & Sons. doi: 10.1002/cpim.13
Kinetics of severe dengue virus infection and development of gut pathology in mice
Pliego Zamora, Adriana, Kim, Jaehyeon, Vajjhala, Parimala R., Thygesen, Sara J., Watterson, Daniel, Modhiran, Naphak, Bielefeldt-Ohmann, Helle and Stacey, Katryn J. (2023). Kinetics of severe dengue virus infection and development of gut pathology in mice. Journal of Virology, 97 (11) ARTN e0125123, e0125123. doi: 10.1128/jvi.01251-23
Curson, James E. B., Liu, Liping, Luo, Lin, Muusse, Timothy W., Lucas, Richard M., Gunther, Kimberley S., Vajjhala, Parimala R., Abrol, Rishika, Jones, Alun, Kapetanovic, Ronan, Stacey, Katryn J., Stow, Jennifer L. and Sweet, Matthew J. (2023). TLR4 phosphorylation at tyrosine 672 activates the ERK/c‐FOS signaling module for LPS‐induced cytokine responses in macrophages. European Journal of Immunology, 53 (7) e2250056, e2250056. doi: 10.1002/eji.202250056
Flow cytometric reporter assays provide robust functional analysis of signaling complexes
Muusse, Timothy W., Lee, Morris Y.L., Kim, Hyoyoung, Parat, Marie-Odile, Nanson, Jeffrey D., Kobe, Bostjan, Vajjhala, Parimala R. and Stacey, Katryn J. (2022). Flow cytometric reporter assays provide robust functional analysis of signaling complexes. Journal of Biological Chemistry, 298 (12) 102666, 102666. doi: 10.1016/j.jbc.2022.102666
Clabbers, Max T. B., Holmes, Susannah, Muusse, Timothy W., Vajjhala, Parimala R., Thygesen, Sara J., Malde, Alpeshkumar K., Hunter, Dominic J. B., Croll, Tristan I., Flueckiger, Leonie, Nanson, Jeffrey D., Rahaman, Md. Habibur, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Yoon, Chun Hong, Zhao, Jingjing, Zatsepin, Nadia A., Abbey, Brian, Sierecki, Emma, Gambin, Yann, Stacey, Katryn J., Darmanin, Connie, Kobe, Bostjan, Xu, Hongyi and Ve, Thomas (2021). MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography. Nature Communications, 12 (1) 2578, 1-14. doi: 10.1038/s41467-021-22590-6
Sagulenko, Vitaliya, Vitak, Nazarii, Vajjhala, Parimala, Vince, James E and Stacey, Katryn J (2017). Caspase-1 is an apical caspase leading to caspase-3 cleavage in the AIM2 inflammasome response, independent of caspase-8. Journal of Molecular Biology, 430 (2), 238-247. doi: 10.1016/j.jmb.2017.10.028
Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling
Ve, Thomas, Vajjhala, Parimala, Hedger, Andrew, Croll, Tristan, DiMaio, Frank, Horsefield, Shane, Yu, Xiong, Lavrencic, Peter, Hassan, Zahid, Morgan, Garry P., Mansell, Ashley, Mobli, Mehdi, O'Carroll, Ailis, Chauvin, Brieuc, Gambin, Yann, Sierecki, Emma, Landsberg, Michael J., Stacey, Katryn, Egelman, Edward H. and Kobe, Bostjan (2017). Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling. Nature Structural and Molecular Biology, 24 (9), 743-751. doi: 10.1038/nsmb.3444
The molecular mechanisms of signaling by cooperative assembly formation in innate immunity pathways
Vajjhala, Parimala R., Ve, Thomas, Bentham, Adam, Stacey, Katryn J. and Kobe, Bostjan (2017). The molecular mechanisms of signaling by cooperative assembly formation in innate immunity pathways. Molecular Immunology, 86, 23-37. doi: 10.1016/j.molimm.2017.02.012
Fu, Tian-Min, Li, Yang, Lu, Alvin, Li, Zongli, Vajjhala, Parimala R., Cruz, Anthony C., Srivastava, Devendra B., DiMaio, Frank, Penczek, Pawel A., Siegel, Richard M., Stacey, Katryn J., Egelman, Edward H. and Wu, Hao (2016). Cryo-EM structure of caspase-8 tandem DED filament reveals assembly and regulation mechanisms of the death-inducing signaling complex. Molecular Cell, 64 (2), 236-250. doi: 10.1016/j.molcel.2016.09.009
The inflammasome adaptor ASC induces procaspase-8 death effector domain filaments
Vajjhala, Parimala R., Lu, Alvin, Brown, Darren L., Pang, Siew Wai, Sagulenko, Vitaliya, Sester, David P., Cridland, Simon O., Hill, Justine M., Schroder, Kate, Stow, Jennifer L., Wu, Hao and Stacey, Katryn J. (2015). The inflammasome adaptor ASC induces procaspase-8 death effector domain filaments. Journal of Biological Chemistry, 290 (49), 29217-29230. doi: 10.1074/jbc.M115.687731
A novel flow cytometric method to assess inflammasome formation
Sester, David P., Thygesen, Sara J., Sagulenko, Vitaliya, Vajjhala, Parimala R., Cridland, Jasmyn A., Vitak, Nazarii, Chen, Kaiwen W., Osborne, Geoffrey W., Schroder, Kate and Katryn J. Stacey (2015). A novel flow cytometric method to assess inflammasome formation. The Journal of Immunology, 194 (1), 455-462. doi: 10.4049/jimmunol.1401110
Vajjhala, Parimala R., Kaiser, Sebastian, Smith, Sarah J., Ong, Qi-Rui, Soh, Stephanie L., Stacey, Katryn J. and Hill, Justine M. (2014). Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Journal of Biological Chemistry, 289 (34), 23504-23519. doi: 10.1074/jbc.M114.553305
AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC
Sagulenko, V., Thygesen, S. J., Sester, D. P., Idris, A., Cridland, J. A., Vajjhala, P. R., Roberts, T., Schroder, K., Vince, J. E., Hill, J. M., Silke, J. and Stacey, K. J. (2013). AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC. Cell Death and Differentiation, 20 (9), 1149-1160. doi: 10.1038/cdd.2013.37
Chen, Kai-En, Richards, Ayanthi A., Caradoc-Davies, Tom T., Vajjhala, Parimala R., Robin, Gautier, Lua, Linda H. L., Hill, Justine M., Schroder, Kate, Sweet, Matthew J., Kellie, Stuart, Kobe, Bostjan and Martin, Jennifer (2013). The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidized. Acta Crystallographica Section D: Biological Crystallography, 69 (5), 774-784. doi: 10.1107/S0907444913001558
Vajjhala, Parimala R., Mirams, Ruth E. and Hill, Justine M. (2012). Multiple Binding Sites on the Pyrin Domain of ASC Protein Allow Self-association and Interaction with NLRP3 Protein. Journal of Biological Chemistry, 287 (50), 41732-41743. doi: 10.1074/jbc.M112.381228
Landsberg, Michael J., Vajjhala, Parimala Rao, Rothnagel, Rosalba, Munn, Alan Leslie and Hankamer, Ben (2009). Three-dimensional structure of AAA ATPase Vps4: Advancing structural insights into the mechanisms of endosomal sorting and enveloped virus budding. Structure, 17 (3), 427-437. doi: 10.1016/j.str.2008.12.020
Vajjhala, P. R., Nguyen , C. H., Landsberg, M. J., Kistler, C., Gan, A. L., King, G. F., Hankamer, B. and Munn, A. L. (2008). The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases.. Febs Journal, 275 (7), 1427-1449. doi: 10.1111/j.1742-4658.2008.06300.x
Host factors in virus budding - Insights from yeast
Vajjhala, P. R. and Munn, A. (2007). Host factors in virus budding - Insights from yeast. Microbiology Australia, May 2007, 44-47.
Thanabalu, T., Rajmohan, R., Meng, L., Ren, G., Vajjhala, P. R. and Munn, A. L. (2007). Verprolin function in endocytosis and actin organization - Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain. FEBS Journal, 274 (16), 4103-4125. doi: 10.1111/j.1742-4658.2007.05936.x
Vps4 regulates a subset of protein interactions at the multivesicular endosome
Vajjhala, P. R., Catchpoole, E., Nguyen, C. H., Kistler, C. and Munn, A. L. (2007). Vps4 regulates a subset of protein interactions at the multivesicular endosome. FEBS Journal, 274 (8), 1894-1907. doi: 10.1111/j.1742-4658.2007.05736.x
The β domain is required for Vps4p oligomerization into a functionally active ATPase
Vajjhala, Parimala R., Wong, Julin S., To, Hui-Yi and Munn, Alan L. (2006). The β domain is required for Vps4p oligomerization into a functionally active ATPase. The Febs Journal, 273 (11), 2357-2373. doi: 10.1111/j.1742-4658.2006.05238.x
The BAR domain proteins: Molding membranes in fission, fusion, and phagy
Ren, G., Vajjhala, P., Lee, J. S., Winsor, B. and Munn, A. L. (2006). The BAR domain proteins: Molding membranes in fission, fusion, and phagy. Microbiology And Molecular Biology Reviews, 70 (1), 37-120. doi: 10.1128/MMBR.70.1.37-120.2006
The Wilms' Tumour Suppressor Protein, WT1, Undergoes CRM1-independent Nucleocytoplasmic Shuttling
Vajjhala, P. R., Macmillan, E., Gonda, T. and Little, M. H. (2003). The Wilms' Tumour Suppressor Protein, WT1, Undergoes CRM1-independent Nucleocytoplasmic Shuttling. FEBS Letters, 554 (1-2), 143-148. doi: 10.1016/S0014-5793(03)01144-X
Cytosolic DNA, HIN-200 proteins and inflammasome activation
Stacey, K. J., Vajjhala, P. R., Sagulenko, V, Thygesen, S., Vitak, N. and Sester, D. P. (2016). Cytosolic DNA, HIN-200 proteins and inflammasome activation. International Congress of Immunology (ICI), Melbourne, VIC Australia, 21-26 August 2016. Weinheim, Germany: Wiley.
Molecular arrangement and activation of procaspases-1 and-8 at inflammasomes
Vajjhala, P., Lu, A., Brown, D., Sagulenko, V, Schroder, K., Stow, J., Wu, H. and Stacey, K. (2016). Molecular arrangement and activation of procaspases-1 and-8 at inflammasomes. ICI 2016 International Congress of Immunology, Melbourne, Australia, 21-26 August 2016. Weinheim, Germany: Wiley.
Expression and characterisation of proteins engineered with Farnesyl groups
Vajjhala, Parimala Rao. (2001). Expression and characterisation of proteins engineered with Farnesyl groups. PhD Thesis, School of Molecular and Microbial Sciences, The University of Queensland.
Mammalian endotoxin: Characterisation of highly inflammatory endogenous material
(2021–2024) NHMRC IDEAS Grants
Toll-like receptor signalling mechanisms
Doctor Philosophy — Associate Advisor
Other advisors:
Structural and functional analysis of TIR domain enzymatic activity
Doctor Philosophy — Associate Advisor
Other advisors:
Molecular analysis of adapter protein interaction with Toll-like receptors
Doctor Philosophy — Associate Advisor
Other advisors:
Molecular analysis of TLR4 signalling
(2023) Doctor Philosophy — Associate Advisor
Other advisors:
Structure of the FADD/MC159 complex reveals the mechanism of DISC assembly and inhibition
(2013) Doctor Philosophy — Associate Advisor