Chris O'Brien is a research fellow at QAAFI leading the cryopreservation program. The program involves the conservation of exceptional species such as avocado and macadamia as well as other plants like Gossia spp which are affected by myrtle rust. The lab focuses on developing and optimizing protocols to store plant germplasm in liquid nitrogen. Storing plants in liquid nitrogen is the safest way to ensure long-term ex situ conservation.
Chris is also leading a project looking at avocado genomics/tissue culture propagation technologies to deliver world-first technology platforms for avocado improvement. This innovative tech-development will address critical challenges of traditional breeding, enabling rapid development of future-smart avocado cultivars.
Journal Article: Incorporating cold pretreatment in cryopreserving shoot tips of sweet myrtle (Gossia fragrantissima)
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Incorporating cold pretreatment in cryopreserving shoot tips of sweet myrtle (Gossia fragrantissima). Cryobiology, 113 104742, 104742. doi: 10.1016/j.cryobiol.2023.104742
Journal Article: Innovative cryopreservation technology for Australian macadamia
Li, Xueying, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Innovative cryopreservation technology for Australian macadamia. Cryobiology, 113 104759, 104759. doi: 10.1016/j.cryobiol.2023.104759
Conference Publication: Cold pretreatment for cryopreservation of an endangered Australian native plant species Gossia fragrantissima
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2022). Cold pretreatment for cryopreservation of an endangered Australian native plant species Gossia fragrantissima. CRYO2022 Joint Meeting of SLTB and Society for Cryobiology, Dublin, Ireland, 19-22 July 2022. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2022.11.045
Gossia gonoclada cryopreservation project
(2022–2026) Logan City Council
Innovating avocado for indoor cropping - Advanced climate-smart production systems
(2022–2025) Advance Queensland Industry Research Fellowships
Cryobiotechnology for conservation of endangered sweet myrtle
(2021) Australian Flora Foundation Inc.
Establishment of avocado genome editing: Dwarfing avocado
Doctor Philosophy
Cryobiotechnology Innovations for Threatened Australian Myrtaceae
Doctor Philosophy
Developing cryopreservation for endangered Myrtaceae
Master Philosophy
Advances in avocado propagation for the sustainable supply of planting materials
Hiti-Bandaralage, Jayeni, Hayward, Alice, O’Brien, Chris, Gleeson, Madeleine, Nak, William and Mitter, Neena (2020). Advances in avocado propagation for the sustainable supply of planting materials. Achieving sustainable cultivation of tropical fruits. (pp. 215-237) Cambridge, United Kingdom: Burleigh Dodds Science Publishing. doi: 10.19103/as.2019.0054.19
Incorporating cold pretreatment in cryopreserving shoot tips of sweet myrtle (Gossia fragrantissima)
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Incorporating cold pretreatment in cryopreserving shoot tips of sweet myrtle (Gossia fragrantissima). Cryobiology, 113 104742, 104742. doi: 10.1016/j.cryobiol.2023.104742
Innovative cryopreservation technology for Australian macadamia
Li, Xueying, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Innovative cryopreservation technology for Australian macadamia. Cryobiology, 113 104759, 104759. doi: 10.1016/j.cryobiol.2023.104759
O'Donohue, Billy, Hiti-Bandaralage, Jayeni, Gleeson, Madeleine, O'Brien, Chris, Harvey, Maggie-Anne, van der Ent, Antony, Pinto Irish, Katherine, Mitter, Neena and Hayward, Alice (2022). Tissue culture tools for selenium hyperaccumulator Neptunia amplexicaulis for development in phytoextraction. Natural Products and Bioprospecting, 12 (1) 28, 1-9. doi: 10.1007/s13659-022-00351-2
Cryopreservation of woody crops: The avocado case
O’Brien, Chris, Hiti-Bandaralage, Jayeni, Folgado, Raquel, Hayward, Alice, Lahmeyer, Sean, Folsom, Jim and Mitter, Neena (2021). Cryopreservation of woody crops: The avocado case. Plants, 10 (5) 934, 934. doi: 10.3390/plants10050934
O'Brien, C., Hiti-Bandaralage, J. C. A., Folgado, R., Lahmeyer, S., Hayward, A., Folsom, J. and Mitter, N. (2020). First report on cryopreservation of mature shoot tips of two avocado (Persea americana Mill.) rootstocks. Plant Cell Tissue and Organ Culture, 144 (1), 103-113. doi: 10.1007/s11240-020-01861-y
O’Brien, C., Hiti-Bandaralage, J., Folgado, R., Lahmeyer, S., Hayward, A., Folsom, J. and Mitter, N. (2020). A method to increase regrowth of vitrified shoot tips of avocado (Persea americana Mill.): First critical step in developing a cryopreservation protocol. Scientia Horticulturae, 266 109305, 109305. doi: 10.1016/j.scienta.2020.109305
Hiti-Bandaralage, Jayeni, Hayward, Alice, O’Brien, Chris, Ahsan, Umair, Gleeson, Madeline, Xue, Yuxin and Mitter, Neena (2020). Phase change related microRNA profiles in the plant regeneration process of avocado through shoot-tip culture. Annals of Advanced Agricultural Sciences, 4 (2). doi: 10.22606/as.2020.42001
Acclimatization of micropropagated mature avocado
Hiti Bandaralage, J.C.A., Hayward, A., O’Brien, C., Beveridge, C. and Mitter, N. (2018). Acclimatization of micropropagated mature avocado. Acta Horticulturae, 1224 (1224), 13-20. doi: 10.17660/ActaHortic.2018.1224.3
Cryopreservation of somatic embryos for avocado germplasm conservation
O'Brien, C., Constantin, M., Walia, A., Yiing, J. L. Y. and Mitter, N. (2016). Cryopreservation of somatic embryos for avocado germplasm conservation. Scientia Horticulturae, 211, 328-335. doi: 10.1016/j.scienta.2016.09.008
Encina, Carlos López, Parisi, Ann, O'Brien, Chris and Mitter, Neena (2014). Enhancing somatic embryogenesis in avocado (Persea americana Mill.) using a two-step culture system and including glutamine in the culture medium. Scientia Horticulturae, 165, 44-50. doi: 10.1016/j.scienta.2013.10.019
Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia
Hacker, Andrew, Puachuay, Meena, Max Shelton, H., Dalzell, Scott, Al Dabbagh, Haider, Jewell, Margaret, van Tran, Thinh, Ngo, The, Zhou, Yi, Halliday, Michael, Giles, Hayley, Loch, Don S., Godwin, Ian D., Fukai, Shu, O'Brien, Chris, Pearce, Will, Anderson, William F. and Lambrides, Christopher J. (2013). Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia. Tropical Grasslands-Forrajes Tropicales, 1 (1), 81-83. doi: 10.17138/TGFT(1)81-83
O'Brien, C.M. and Drew, R.A. (2010). Marker-assisted hybridisation and backcrossing between Vasconcellea species and Carica papaya for prsv-p resistance. Acta Horticulturae (859), 361-368. doi: 10.17660/actahortic.2010.859.43
O'Brien, C. M. and Drew, R. A. (2009). Potential for using Vasconcellea parviflora as a bridging species in intergeneric hybridisation between V. pubescens and Carica papaya. Australian Journal of Botany, 57 (7), 592-601. doi: 10.1071/bt09111
Drew, R.A., Siar, S.V., O'Brien, C.M., Magdalita, P.M. and Sajise, A.G.C. (2006). Breeding for Papaya ringspot virus resistance in Carica papaya via hybridisation with Vasconcellea quercifolia. Australian Journal of Experimental Agriculture, 46 (3), 413-418. doi: 10.1071/ea04247
Drew, R.A., Siar, S.V., O'Brien, C.M. and Sajise, A.G.C. (2006). Progress in backcrossing between Carica papaya x Vasconcellea quercifolia intergeneric hybrids and C. papaya. Australian Journal of Experimental Agriculture, 46 (3), 419-424. doi: 10.1071/ea04248
Cryopreservation of papaya germplasm
Azimi, M., O'Brien, C., Ashmore, S. and Drew, R. (2005). Cryopreservation of papaya germplasm. Acta Horticulturae (692), 43-50. doi: 10.17660/actahortic.2005.692.4
Drew, R.A., Siar, S.V., Villegas, V.N., O´Brien, C.M. and Sajise, A.G.C. (2005). Development of PRSV-P resistant Carica papaya genotypes by introgression of genes from wild Vasconcella species. Acta Horticulturae (694), 73-77. doi: 10.17660/actahortic.2005.694.8
Van Droogenbroeck, B., Maertens, I., Haegeman, A., Kyndt, T., O'Brien, C., Drew, R.A. and Gheysen, G. (2005). Maternal inheritance of cytoplasmic organelles in intergeneric hybrids of Carica papaya L. and Vasconcellea spp. (Caricaceae Dumort., Brassicales). Euphytica, 143 (1-2), 161-168. doi: 10.1007/s10681-005-3156-0
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2022). Cold pretreatment for cryopreservation of an endangered Australian native plant species Gossia fragrantissima. CRYO2022 Joint Meeting of SLTB and Society for Cryobiology, Dublin, Ireland, 19-22 July 2022. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2022.11.045
Innovative cryopreservation technology for Australian macadamia
Li, Xueying, O’brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2022). Innovative cryopreservation technology for Australian macadamia. CRYO2022 59th Annual Meeting of the Society for Cryobiology, Dublin, Ireland, 19-22 July 2022. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2022.11.198
O’Brien, C., Hiti-Bandaralage, J.C.A., Folgado, R., Lahmeyer, S., Hayward, A. and Mitter, N. (2020). Developing a cryopreservation protocol for avocado (Persea americana Mill.) apical shoot tips using different antioxidants. International Horticultural Congress, Istanbul, Turkey, 12-16 August 2018. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/actahortic.2020.1285.3
Avocado micropropagation: growth in leaps and bounds for a clonal rootstock propagation solution
Hiti Bandaralage, J. C. A., Hayward, A., O'Brien, C., Beveridge, C. and Mitter, N. (2019). Avocado micropropagation: growth in leaps and bounds for a clonal rootstock propagation solution. Fifth International Conference of the IUFRO Working Party Unit 2.09.02 “Somatic embryogenesis and other vegetative propagation technologies”, Coimbra, Portugal, 10 - 15 September 2018. Coimbra, Rua Bernardino: João Duarte, Unipessoal.
O'Brien, Chris, Bandaralage, Jayeni Hiti, Folgado, Raquel, Lahmeyer, Sean, Hayward, Alice and Mitter, Neena (2019). The Use Of Sucrose Pre-Culture For Cryopreservation Of Avocado (Persea Americana Mill.) Cultivar ‘Velvick’. 56th Annual Meeting of the Society for Cryobiology CRYO 2019, San Diego, CA United States, 22-25 July 2019. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2019.10.056
Developing a cryopreservation protocol for avocado (Persea Americana Mill.) shoot tips
O’Brien, Chris, Hiti Bandaralage, Jayeni C. A., Folgado, Raquel, Lahmeyer, Sean, Hayward, Alice and Mitter, Neena (2018). Developing a cryopreservation protocol for avocado (Persea Americana Mill.) shoot tips. CRYO2018, Madrid, Spain, 10-13 July 2018. Maryland Heights, MO United States: Elsevier. doi: 10.1016/j.cryobiol.2018.10.199
Temporal profile of juvenility-associated microRNAs during tissue culture of avocado
Hiti Bandaralage, J. C.A., Hayward, A., O’Brien, C. and Mitter, N. (2018). Temporal profile of juvenility-associated microRNAs during tissue culture of avocado. International Symposia on Tropical and Temperate Horticulture - ISTTH2016, Cairns, QLD, Australia, 20-25 November 2016. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2018.1205.97
Micropropagation: The Future of Clonal Propagation of Avocado
Hiti Bandalarage, Jayeni , Hayward, Alice , O'Brien, Christopher and Mitter, Neena (2017). Micropropagation: The Future of Clonal Propagation of Avocado. International Tropical Agriculture Conference, Brisbane, QLD, Australia, 20-22 November 2017. Brisbane, QLD, Australia: TropAg.
Cryopreservation of avocado (Persea americana Mill.) using somatic embryos
O'Brien, C., Parisi, A., Yuan, J. Lim Yiing, Constantin, M. and Mitter, N. (2016). Cryopreservation of avocado (Persea americana Mill.) using somatic embryos. XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques, Brisbane, QLD., Australia, 17-22 August 2014. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2016.1113.39
Factors affecting avocado shoot health in culture
Mohd Rodzi, A., Hayward, A., Hiti Bandaralage, J., O'Brien, C., Gleeson, M. and Mitter, N. (2015). Factors affecting avocado shoot health in culture. VIII World Avocado Congress, Lima, Peru, 13- 18 September 2015. Lima Peru: World Avocado Congress.
Gibberellin and cytokinin in synergy for a rapid nodal multiplication system of avocado
Hiti Bandaralage, J., Hayward, A., O'Brien, C. and Mitter, N. (2015). Gibberellin and cytokinin in synergy for a rapid nodal multiplication system of avocado. VIII World Avocado Congress, Lima, Peru, 13- 18 September 2015. Lima Peru: World Avocado Congress.
Lambrides, Christopher J., Zhou, Yi, Fukai, Shu, Jewell, Margaret, Loch, Don, Godwin, Ian, Holton, Tim, Innes, David, Frere, Celine, Hanna, Wayne, Ye, Changrong, Van Tran, Thinh, Le, Thu, Cao, Ninh, Al Dabbagh, Haider, O'Brien, Chris, Roche, Matt, Fullelove, Garry, Ballard, Emma, Kearns, Ryan, Pearce, Will, Hacker, Andrew, Puachuay, Meena, Shelton, Max, Dalzell, Scott, Anderson, Bill, Harris-Shultz, Karen and Cremer, Julia (2013). Ecoturf - a case study on genetic variation and agronomic potential of bermudagrass (cynodon spp.) germplasm collected from Australian biodiversity. ITRC 2013: 12th International Turfgrass Research Conference, Beijing, China, 14-19 July 2013. Overland Park, KS, USA: Intertec Publishing.
Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia
Hacker, Andrew, Puachuay, Meena, Shelton, H. Max, Dalzell, Scott, Al Dabbagh, Haider, Jewell, Margaret, Tran, Thinh Van, Ngo, The, Zhou, Yi, Halliday, Michael, Giles, Hayley, Loch, Don S., Godwin, Ian D., Fukai, Shu, O'Brien, Chris, Pearce, Will, Anderson, William F. and Lambrides, Christopher J. (2013). Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia. IGC2013: 22nd International Grassland Congress 2013, Sydney, NSW, Australia, 15-19 September, 2013. Santiago de Cali, Colombia: Centro Internacional de Agricultura Tropical.
Ashmore, S. E., Drew, R. A., O'Brien, C. and Parisi, A. (2009). Cryopreservation of Papaya (Carica papaya L.) Seed: Overcoming Dormancy and Optimizing Seed Desiccation and Storage Conditions. 1st International Symposium on Biotechnology of Fruit Species - BIOTECHFRUIT, Dresden, Germany, 14 July 2009. Leuven, Belgium: International Society of Horticultural Science.
Zhou, Y., Fukai, S., Cao, N., Ye, C. R., Le, T., Loch, D., Godwin, I., O'Brien, C., Dunne, R., Giles, H., Wilson, M., Halliday, M. and Lambrides, C. (2009). The use of infrared thermometer to detect drought resistance of green couch grass (Cynodon sp.) genotypes collected from the world'd driest inhabited continent. The 3rd International Conference on Integrated Approaches to Improve Crop Production Under Drought Prone Environments, Shanghai, China, 11-16 October 2009.
Drew, R., Siar, S. V., Dillon, S., Ramage, C., O'Brien, C. and Sajise, A. G. C. (2007). Intergeneric hybridisation between Carica papaya and wild Vasconcellea species and identification of a PRSV-P resistance gene. International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species, Daytona Beach, FL United States, 10-14 October 2005. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2007.738.14
Dillon, S., O'Brien, C. M., Drew, R. A. and Ramage, C. (2006). Application of SCAR markers linked to a putative PRSV-P resistance locus for assisted breeding of resistant C-papaya cultivars. 5th International Symposium on In Vitro Culture and Horticultural Breeding, Debrecen, Hungary, 12-17 September 2004. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2006.725.87
Papaya ringspot virus in Australia and the development of virus resistant plants
Drew, R., Persley, D., O'Brien, C. and Bateson, M. (2005). Papaya ringspot virus in Australia and the development of virus resistant plants. 2nd International Symposium on Biotechnology of Tropical and Subtropical Species, Taipei, Taiwan, 5-9 November 2001. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2005.692.12
Development of Carica interspecific hybrids
Drew, R.A., O'Brien, C.M. and Magdalita, P.M. (1998). Development of Carica interspecific hybrids. International Symposium on Biotechnology of Tropical and Subtropical Species, Brisbane, QLD Australia, 29 September - 3 October 1997. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.1998.461.31
Cryopreservation of avocado shoot tips for the conservation of Persea germplasm
O'Brien, Christopher Michael (2021). Cryopreservation of avocado shoot tips for the conservation of Persea germplasm. PhD Thesis, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland. doi: 10.14264/7b83f15
Gossia gonoclada cryopreservation project
(2022–2026) Logan City Council
Innovating avocado for indoor cropping - Advanced climate-smart production systems
(2022–2025) Advance Queensland Industry Research Fellowships
Cryobiotechnology for conservation of endangered sweet myrtle
(2021) Australian Flora Foundation Inc.
Cryobiotechnology for conservation of endangered Sweet Myrtle
(2020–2022) Australian Flora Foundation Inc.
Establishment of avocado genome editing: Dwarfing avocado
Doctor Philosophy — Principal Advisor
Other advisors:
Cryobiotechnology Innovations for Threatened Australian Myrtaceae
Doctor Philosophy — Associate Advisor
Other advisors:
Developing cryopreservation for endangered Myrtaceae
Master Philosophy — Associate Advisor
Other advisors:
Novel Propagation and Conservation Technologies for Australian Macadamia
Doctor Philosophy — Associate Advisor
Other advisors:
Innovating Cryopreservation for Conservation of Endangered Gossia gonoclada
Doctor Philosophy — Associate Advisor
Other advisors:
Novel propagation and conservation technologies for Australian macadamia
Doctor Philosophy — Associate Advisor
Other advisors: