The University of Queensland

High-throughput production of oxidized animal toxins in Escherichia coli

Duhoo, Yoan, Sequeira, Ana Filipa, Saez, Natalie J, Turchetto, Jeremy, Ramond, Laurie, Peysson, Fanny, Brás, Joana L A, Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M G A and Vincentelli, Renaud (2019). High-throughput production of oxidized animal toxins in Escherichia coli. High-Throughput Protein Production and Purification. (pp. 165-190) edited by Renaud Vincentelli. New York, NY, United States: Humana. doi: 10.1007/978-1-4939-9624-7_7

A Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli

Saez, Natalie J., Cristofori-Armstrong, Ben, Anangi, Raveendra and King, Glenn F. (2017). A Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli. In Heterologous Gene Expression in E. coli Methods and Protocols (pp. 155-180) New York, NY United States: Humana Press. doi:10.1007/978-1-4939-6887-9_10

High-throughput expression screening and purification of recombinant proteins in E-coli

Saez, Natalie J. and Vincentelli, Renaud (2014). High-throughput expression screening and purification of recombinant proteins in E-coli. Structural genomics: General applications. (pp. 33-53) edited by Yu Wai Chen. New York United States: Humana Press. doi: 10.1007/978-1-62703-691-7_3

Hi1a Improves Sensorimotor Deficit following Endothelin-1-Induced Stroke in Rats but Does Not Improve Functional Outcomes following Filament-Induced Stroke in Mice

Knezic, Adriana, Budusan, Elena, Saez, Natalie J., Broughton, Brad R. S., Rash, Lachlan D., King, Glenn F., Widdop, Robert E. and McCarthy, Claudia A. (2024). Hi1a Improves Sensorimotor Deficit following Endothelin-1-Induced Stroke in Rats but Does Not Improve Functional Outcomes following Filament-Induced Stroke in Mice. ACS Pharmacology & Translational Science. doi: 10.1021/acsptsci.3c00328

Acid-sensing ion channel 1a blockade reduces myocardial injury in rodent models of myocardial infarction

Redd, Meredith A, Yoshikawa, Yusuke, Khan, Nemat, Waqar, Maleeha, Saez, Natalie J, Outhwaite, Jennifer E, Russell, Jake S, Hanna, Amy D, Chiu, Han S, Er, Sing Yan, Butcher, Neville J, Mardon, Karine, Fraser, John F, Smythe, Mark L, Rash, Lachlan D, Thomas, Walter G, King, Glenn F, Reichelt, Melissa E and Palpant, Nathan J (2023). Acid-sensing ion channel 1a blockade reduces myocardial injury in rodent models of myocardial infarction. European Heart Journal. doi: 10.1093/eurheartj/ehad793

Acute inhibition of acid sensing ion channel 1a after spinal cord injury selectively affects excitatory synaptic transmission, but not intrinsic membrane properties, in deep dorsal horn interneurons

Foster, Victoria S., Saez, Natalie, King, Glenn F. and Rank, Michelle M. (2023). Acute inhibition of acid sensing ion channel 1a after spinal cord injury selectively affects excitatory synaptic transmission, but not intrinsic membrane properties, in deep dorsal horn interneurons. PLoS One, 18 (11 November) e0289053, 1-23. doi: 10.1371/journal.pone.0289053

Evaluation of peptide ligation strategies for the synthesis of the bivalent acid-sensing ion channel inhibitor Hi1a

Tran, Hue N. T., Budusan, Elena, Saez, Natalie J., Norman, Alexander, Tucker, Isaac J., King, Glenn F., Payne, Richard J., Rash, Lachlan D., Vetter, Irina and Schroeder, Christina I. (2023). Evaluation of peptide ligation strategies for the synthesis of the bivalent acid-sensing ion channel inhibitor Hi1a. Organic Letters, 25 (24), 4439-4444. doi: 10.1021/acs.orglett.3c01346

Genetic or pharmacological ablation of acid-sensing ion channel 1a (ASIC1a) is not neuroprotective in a mouse model of spinal cord injury

Foster, Victoria, Saez, Natalie, Gillespie, Ellen, Jogia, Trisha, Reid, Chantelle, Maljevic, Snezana, Jung, Woncheol, Lao, Hong W., Ruitenberg, Marc Jan and King, Glen (2023). Genetic or pharmacological ablation of acid-sensing ion channel 1a (ASIC1a) is not neuroprotective in a mouse model of spinal cord injury. Journal of Neurotrauma. doi: 10.1089/neu.2022.0295

A peptide toxin in ant venom mimics vertebrate EGF-like hormones to cause long-lasting hypersensitivity in mammals

Eagles, David A., Saez, Natalie J., Krishnarjuna, Bankala, Bradford, Julia J., Chin, Yanni K.-Y., Starobova, Hana, Mueller, Alexander, Reichelt, Melissa E., Undheim, Eivind A. B., Norton, Raymond S., Thomas, Walter G., Vetter, Irina, King, Glenn F. and Robinson, Samuel D. (2022). A peptide toxin in ant venom mimics vertebrate EGF-like hormones to cause long-lasting hypersensitivity in mammals. Proceedings of the National Academy of Sciences, 119 (7) e2112630119. doi: 10.1073/pnas.2112630119

Strategies for heterologous expression, synthesis, and purification of animal venom toxins

Rivera-de-Torre, Esperanza, Rimbault, Charlotte, Jenkins, Timothy P., Sørensen, Christoffer V., Damsbo, Anna, Saez, Natalie J., Duhoo, Yoan, Hackney, Celeste Menuet, Ellgaard, Lars and Laustsen, Andreas H. (2022). Strategies for heterologous expression, synthesis, and purification of animal venom toxins. Frontiers in Bioengineering and Biotechnology, 9 811905, 811905. doi: 10.3389/fbioe.2021.811905

Total synthesis of the spider-venom peptide Hi1a

Duggan, Nisharnthi M., Saez, Natalie J., Clayton, Daniel, Budusan, Elena, Watson, Emma E., Tucker, Isaac J., Rash, Lachlan D., King, Glenn F. and Payne, Richard J. (2021). Total synthesis of the spider-venom peptide Hi1a. Organic Letters, 23 (21) acs.orglett.1c03112, 8375-8379. doi: 10.1021/acs.orglett.1c03112

Therapeutic inhibition of acid sensing ion channel 1a recovers heart function after ischemia-reperfusion injury

Redd, Meredith A., Scheuer, Sarah E., Saez, Natalie J., Yoshikawa, Yusuke, Chiu, Han Sheng, Gao, Ling, Hicks, Mark, Villanueva, Jeanette E., Joshi, Yashutosh, Chow, Chun Yuen, Cuellar-Partida, Gabriel, Peart, Jason N., See Hoe, Louise E., Chen, Xiaoli, Sun, Yuliangzi, Suen, Jacky Y., Hatch, Robert J., Rollo, Ben, Xiao, Di, Alzubaidi, Mubarak A.H., Maljevic, Snezana, Quaife-Ryan, Gregory A., Hudson, James E., Porrello, Enzo R., White, Melanie Y., Cordwell, Stuart J., Fraser, John F., Petrou, Steven, Reichelt, Melissa E. ... Palpant, Nathan J. (2021). Therapeutic inhibition of acid sensing ion channel 1a recovers heart function after ischemia-reperfusion injury. Circulation, 144 (12), 947-960. doi: 10.1161/circulationaha.121.054360

A venomics approach coupled to high-throughput toxin production strategies identifies the first venom-derived melanocortin receptor agonists

Reynaud, Steve, Ciolek, Justyna, Degueldre, Michel, Saez, Natalie J., Sequeira, Ana Filipa, Duhoo, Yoan, Brás, Joana L A, Meudal, Hervé, Cabo Díez, Miguel, Fernández Pedrosa, Victoria, Verdenaud, Marion, Boeri, Julia, Pereira Ramos, Oscar, Ducancel, Frédéric, Vanden Driessche, Margot, Fourmy, Rudy, Violette, Aude, Upert, Grégory, Mourier, Gilles, Beck-Sickinger, Annette G., Mörl, Karin, Landon, Céline, Fontes, Carlos M. G. A., Miñambres Herráiz, Rebeca, Rodríguez de la Vega, Ricardo C., Peigneur, Steve, Tytgat, Jan, Quinton, Loïc, De Pauw, Edwin ... Gilles, Nicolas (2020). A venomics approach coupled to high-throughput toxin production strategies identifies the first venom-derived melanocortin receptor agonists. Journal of Medicinal Chemistry, 63 (15), 8250-8264. doi: 10.1021/acs.jmedchem.0c00485

The antitrypanosomal diarylamidines, diminazene and pentamidine, show anthelmintic activity against Haemonchus contortus in vitro

Nixon, Samantha A., Saez, Natalie J., Herzig, Volker, King, Glenn F. and Kotze, Andrew C. (2019). The antitrypanosomal diarylamidines, diminazene and pentamidine, show anthelmintic activity against Haemonchus contortus in vitro. Veterinary Parasitology, 270, 40-46. doi: 10.1016/j.vetpar.2019.05.008

The modulation of acid-sensing ion channel 1 by PcTx1 is pH-, subtype- and species-dependent: importance of interactions at the channel subunit interface and potential for engineering selective analogues

Cristofori-Armstrong, Ben, Saez, Natalie J., Chassagnon, Irène R., King, Glenn F. and Rash, Lachlan D. (2019). The modulation of acid-sensing ion channel 1 by PcTx1 is pH-, subtype- and species-dependent: importance of interactions at the channel subunit interface and potential for engineering selective analogues. Biochemical Pharmacology, 163, 381-390. doi: 10.1016/j.bcp.2019.03.004

Versatile spider venom peptides and their medical and agricultural applications

Saez, Natalie J. and Herzig, Volker (2019). Versatile spider venom peptides and their medical and agricultural applications. Toxicon, 158, 109-126. doi: 10.1016/j.toxicon.2018.11.298

Inhibition of acid-sensing ion channels by diminazene and APETx2 evoke partial and highly variable antihyperalgesia in a rat model of inflammatory pain

Lee, Jia Yu Peppermint, Saez, Natalie J., Cristofori-Armstrong, Ben, Anangi, Raveendra, King, Glenn F., Smith, Maree T. and Rash, Lachlan D. (2018). Inhibition of acid-sensing ion channels by diminazene and APETx2 evoke partial and highly variable antihyperalgesia in a rat model of inflammatory pain. British Journal of Pharmacology, 175 (12), 2204-2218. doi: 10.1111/bph.14089

ArachnoServer 3.0: an online resource for automated discovery, analysis and annotation of spider toxins

Pineda, Sandy S., Chaumeil, Pierre-Alain, Kunert, Anne, Kaas, Quentin, Thang, Mike W. C., Le, Lien, Nuhn, Michael, Herzig, Volker, Saez, Natalie J., Cristofori-Armstrong, Ben, Anangi, Raveendra, Senff, Sebastian, Gorse, Dominique and King, Glenn F. (2017). ArachnoServer 3.0: an online resource for automated discovery, analysis and annotation of spider toxins. Bioinformatics, 34 (6), 1074-1076. doi: 10.1093/bioinformatics/btx661

Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

Sequeira, Ana Filipa, Turchetto, Jeremy, Saez, Natalie J., Peysson, Fanny, Ramond, Laurie, Duhoo, Yoan, Blemont, Marilyne, Fernandes, Vânia O., Gama, Luís T., Ferreira, Luís M. A., Guerreiro, Catarina I. P. I., Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M. G. A. and Vincentelli, Renaud (2017). Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli. Microbial Cell Factories, 16 (1) 4, 4. doi: 10.1186/s12934-016-0618-0

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

Turchetto, Jeremy, Sequeira, Ana Filipa, Ramond, Laurie, Peysson, Fanny, Bras, Joana L. A., Saez, Natalie J., Duhoo, Yoan, Blemont, Marilyne, Guerreiro, Catarina I. P. D., Quinton, Loic, Pauw, Edwin de, Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M. G. A. and Vincentelli, Renaud (2017). High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery. Microbial Cell Factories, 16 (1) 6, 6. doi: 10.1186/s12934-016-0617-1

Molecular dynamics and functional studies define a hot spot of crystal contacts essential for PcTx1 inhibition of acid-sensing ion channel 1a

Saez, Natalie J., Deplazes, Evelyne, Cristofori-Armstrong, Ben, Chassagnon, Irene R., Lin, Xiaozhen, Mobli, Mehdi, Mark, Alan E., Rash, Lachlan D. and King, Glenn F. (2015). Molecular dynamics and functional studies define a hot spot of crystal contacts essential for PcTx1 inhibition of acid-sensing ion channel 1a. British Journal of Pharmacology, 172 (20), 4985-4995. doi: 10.1111/bph.13267

High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli

Saez, Natalie J., Nozach, Herve, Blemont, Marilyne and Vincentelli, Renaud (2014). High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli. Journal of Visualized Experiments, 1091 (89) e51464, 33-53. doi: 10.3791/51464

Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli

Klint, Julie K., Senff, Sebastian, Saez, Natalie J., Seshadri, Radha, Lau, Ho Yee, Bende, Nira J., Undheim, Eivind A. B., Rash, Lachlan D., Mobli, Mehdi and King, Glenn F. (2013). Production of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli. PLoS One, 8 (5) e63865, e63865.1-e63865.12. doi: 10.1371/journal.pone.0063865

High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

Nozach, Hervé, Fruchart-Gaillard, Carole, Fenaille, François, Beau, Fabrice, Ramos, Oscar Henrique Pereira, Douzi, Badreddine, Saez, Natalie J., Moutiez, Mireille, Servent, Denis, Gondry, Muriel, Thaï, Robert, Cuniasse, Philippe, Vincentelli, Renaud and Dive, Vincent (2013). High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microbial Cell Factories, 12 (37) 37, 1-16. doi: 10.1186/1475-2859-12-37

A dynamic pharmacophore drives the interaction between psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a

Saez, Natalie J., Mobli, Mehdi, Bieri, Michael, Chassagnon, Irene R., Malde, Alpeshkumar K., Gamsjaeger, Roland, Mark, Alan E., Gooley, Paul R., Rash. Lachlan D. and King, Glenn F. (2011). A dynamic pharmacophore drives the interaction between psalmotoxin-1 and the putative drug target acid-sensing ion channel 1a. Molecular Pharmacology, 80 (5), 796-808. doi: 10.1124/mol.111.072207

Possible roles for Munc18-1 domain 3a and Syntaxin1 N-peptide and C-terminal anchor in SNARE complex formation

Hu, Shu-Hong, Christie, Michelle P., Saez, Natalie J., Latham, Catherine F., Jarrott, Russell, Lua, Linda H. L., Collins, Brett M. and Martin, Jennifer L. (2011). Possible roles for Munc18-1 domain 3a and Syntaxin1 N-peptide and C-terminal anchor in SNARE complex formation. Proceedings of the National Academy of Sciences of the United States of America, 108 (3), 1040-1045. doi: 10.1073/pnas.0914906108

Spider-venom peptides as therapeutics

Seaz, Natalie J., Senff, Sebastian, Jensen, Jonas E., Er, Sing Yan, Herzig, Volker, Rash, Lachlan D. and King, Glenn F. (2010). Spider-venom peptides as therapeutics. Toxins, 2 (12), 2851-2871. doi: 10.3390/toxins2122851

A spider-venom peptide for preventing ischemic injuries of the heart: application to heart transplant and myocardial infarction

Redd, M. A., Scheuer, S. E., Saez, N. J., Macdonald, P. S., Palpant, N. J. and King, G. F. (2020). A spider-venom peptide for preventing ischemic injuries of the heart: application to heart transplant and myocardial infarction. Venom Week, Gainesville, FL, United States, 4-7 March, 2020. Oxford, United Kingdom : Elsevier.

Characterising the molecular basis of the interaction between the putative drug target ASIC1a and π-TRTX-Pc1a

Saez, Natalie Jose (2013). Characterising the molecular basis of the interaction between the putative drug target ASIC1a and π-TRTX-Pc1a. PhD Thesis, Institute for Molecular Bioscience, The University of Queensland.