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Gene expression profiling and de novo transcriptome sequencing using geneballs (2003–2006)

Abstract:
The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics for use in real world applications. The project has two aspects. First, relatively small directed cDNA-bead libraries will be compared to known low-density cDNA microarrays to validate the technique for utility in gene expression profiling. Secondly, large libraries containing short oligonucleotide sequences will be used for de novo sequencing of a complete transcriptome. Proof-of-concept in either case will pave the way for many genomic applications and catapult the technology to blockbuster status.
Grant type:
ARC Linkage Projects
Researchers:

  • Professor David Hume

    Professor
    Mater Research Institute-UQ
    Faculty of Medicine

  • Professor Gwendolyn Lawrie

    Professor
    School of Chemistry and Molecular Biosciences
    Faculty of Science

  • Professor Matt Sweet

    NHMRC Leadership Fellow - GL
    Institute for Molecular Bioscience
    Affiliate NHMRC Leadership Fellow
    School of Chemistry and Molecular Biosciences
    Faculty of Science
Funded by:
Australian Research Council