Single molecule spectroscopy-guided design of thermostable industrial enzymes (2012–2015)
- Abstract:
- Recombinant proteins with modified properties are the backbone of biotechnology. To be useful in industrial settings, the stability of proteins at higher temperatures typically requires improvement. Thermostability is a property that is difficult to select for in most of the currently available selection systems. Here we propose to use a combination of cell-free protein expression and on-microfluidic chip single molecule analysis to test thermostability of proteins. A high-throughput version of this tool will be used to develop thermostabilised versions of phytase, xylanase and beta-glucanase. Such enzymes will allow manufacturing of a new generation of functionalised feedstock and improve competitiveness of Australian agriculture.
- Grant type:
- ARC Linkage Projects
- Researchers:
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Emeritus Professor
Australian Institute for Bioengineering and Nanotechnology
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- Funded by:
- Australian Research Council
